![]() ![]() The predicted isoelectric point of Dof proteins varied from 4.77 to 9.92 ( S1 Table). Correspondingly, the molecular weights were mainly distributed from 18318.89 Da to 59589.04 Da. The length of these cotton Dof protein sequences mainly centered on the range of 164~543 amino acid residues. The gene number in tetraploid cotton is almost twice that of diploid cotton, and is more than in rice (30 Dof genes) and Arabidopsis (36 Dof genes). In the present study, we identified 115, 116, 55 and 56 Dof genes from G. We used a whole-genome scan to identify genes that encode proteins containing the Dof domain by both BLASTP and HMMER. Genome-wide identification and characterization of Dof gene family in cotton This study will provide valuable clues for functional characterization of Dof gene family in cotton. In the present study, we performed a comprehensive analysis of cotton Dof genes, including their gene structure, motif compositions, chromosome distribution, duplication patterns and expression profiles. Whole genome sequenes of two cultivated tetraploid species, upland cotton ( Gossypium hirsutum) and island cotton ( Gossypium barbadense), and two diploid species Gossypium arboretum and Gossypium raimondii provided an opportunity to reveal the traits of cotton Dof gene family at genome-wide level. ![]() Because of the importance of Dof gene in various physiological processes, it would be necessary to perform a genome-wide identification and comparative analysis of Dof family in different cotton species. Furthermore, the Dof gene family of Gossypium hirsutum was also studied. The Dof gene family has been extensively studied in many plant species, such as Arabidopsis thaliana, Oryza sativa, Jatropha curcas and Setaria italic. In recent years, as more and more plant genome data have been published, genome-wide analysis has become an very effective way for gene function prediction from a large family of genes and there are an increasing number of reports about cotton gene families. All tetraploid cotton species came from interspecific hybridization between the A-genome species and the D-genome species. Cotton is commonly grouped into eight diploid genomic groups, designated A-G and K, and one tetraploid genomic group, namely AD. Current understanding recognizes more than 50 species within the cotton genus, with both diploid and polyploid members. Furthermore, cotton is also an excellent model system for studying polyploidization and cell elongation. Ĭotton ( Gossypium) is one of the most important agronomic genera in the world. In spite of high level homology in the Dof domain, the rest of the sequences are divergent, coinciding with their expected diverse functions. Dof specifically bind AAAG sequences of plant gene promoters with the Dof DNA-binding domain. The Dof (DNA-binding one zinc finger) is a plant-specific transcription factor having multiple roles such as carbon assimilation, light-mediated regulation, seed maturation and germination. Transcription factors play a very vital role in gene regulation at transcriptional level. Overall, our results could provide valuable information for better understanding the evolution of cotton Dof genes, and lays a foundation for future investigation in cotton. Analysis of RNA-Seq data indicated that cotton Dof gene expression levels varied across different tissues and in response to different abiotic stress. Genome comparison revealed that segmental duplication may have played crucial roles in the expansion of the cotton Dof gene family, and tandem duplication also played a minor role. Those genes were unevenly distributed across 13/26 chromosomes of the cotton. 115, 116, 55 and 56 Dof genes were identified respectively and all of the genes contain a sequence encoding the Dof DNA-binding domain. In this study, we performed a genome-wide screening and characterization of the Dof gene family within two tetraploid species Gossypium barbadense, Gossypium hirsutum, and two diploid species Gossypium arboreum, Gossypium raimondii. The Dof (DNA-binding one zinc finger) transcription factor family is a representative of plant-specific classes of transcription factors. ![]()
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